In this application note, we will show that the Wavelet Transform can be used as an efficient method for the removal of diagonal peaks in 2D homonuclear NMR experiments. Selectivity: The width of the suppressed signal can be adjusted so that the peaks close to it are recovered without peak distortion of the peak shape and intensity.Recently, the Wavelet Transform has been proved to be extremely effective to the suppression of on-resonance solvent signals satisfying two fundamental criteria : On the other hand, post processing methods, usually accomplished by means of modified high-pass frequency convolution filters, often distort peak intensities and baseline close to the diagonal peaks. Modifications of the pulse sequences require more complicated acquisitions schemes and often decrease the experiment sensitivity. Different approaches to obtain diagonal-free 2D NMR spectra have been recently reported ranging from modifications of the pulse sequences to post processing techniques. It is not uncommon that the diagonal obscures close cross peaks so removing it will facilitate a more accurate analysis of the 2D NMR experiment of interest. Among the many 2D NMR experiments, the so-called homonuclear experiments are characterized by having diagonal peaks which merely provide the same information as the conventional 1D experiment and cross peaks which are the important diagnostic feature. Multidimensional NMR spectroscopy has expanded dramatically in recent years and is now considered the most powerful tool for the study of structure, dynamics, and interactions of both low molecular weight compounds and biomolecules.
#MESTRENOVA MIRROR TWO NMR PDF#
Note: A PDF version of this article can be downloaded from the Mestrelab Research site as an Application note of June 25, 2007. The performance of the approach is illustrated by applying it to a phase-sensitive TOCSY spectrum of strychnine. First released February 20, 2008Ībstract: It is shown that Wavelet Transform can be used to efficiently remove interfering diagonal peaks in 2D homonuclear NMR experiments. Applied to the diagonal suppression of 2D NMR data setsīy Carlos Cobas, Mestrelab Research, Santiago de Compostela, Spain